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dc.contributor.authorGuney, Sevin
dc.contributor.authorDincer, Sibel
dc.contributor.authorGoktas, Guleser
dc.contributor.authorTake-Kaplanoglu, Gulnur
dc.date.accessioned2020-10-14T08:46:26Z
dc.date.available2020-10-14T08:46:26Z
dc.date.issued2019
dc.identifier10.3906/sag-1810-51
dc.identifier.issn1300-0144
dc.identifier.urihttp://hdl.handle.net/20.500.12591/228
dc.description.abstractBackground/aim: The purpose of the present study was to explore the neuroprotective role of delta opioid receptors (DOR) in the rat cortex in hypoxic preconditioning. Materials and methods: Rats were randomly divided into 8 groups: control (C), sham (5), hypoxic preconditioning (PC), severe hypoxia (SH), PC + SH, PC + SH + Saline (PS), PC + SH + DPDPE (DPDPE, selective DOR agonise), PC + SH + NT (NT, Naltrindole, selective DOR antagonist). Drugs were administered intracerebroventrically. Twenty four h after the end of 3 consecutive days of PC (10\% O-2 , 2 h/day), the rats were subjected to severe hypoxia (7\% O-2 for 3 h). Bcl-2 and cyt-c were measured by western blot, and caspase-3 was observed immunohistochemically. Results: Bcl-2 expressions in the PC group were higher than in control, SH, and PC + SH groups. Even though there were no significant differences between the groups in terms of cyt-c levels, caspase-3 immunoreactivity of cortical neurons and glial cells in the severe hypoxia and NT groups were higher than in the control, sham, and hypoxic preconditioning groups. DPDPE administration diminished caspase-3 immunoreactivity compared with all of the severe hypoxia groups. Conclusions: These results suggest that cortical cells are resistant to apoptosis via increased expression of Bcl-2 and decreased immunoreactivity of caspase-3 in the cortex, and that DOR is involved in neuroprotection induced by hypoxic preconditioning via the caspase-3 pathway in cortical neurons.
dc.sourceTURKISH JOURNAL OF MEDICAL SCIENCES
dc.titleNeuroprotective role of delta opioid receptors in hypoxic preconditioning


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