• Türkçe
    • English
  • English 
    • Türkçe
    • English
  • Login
View Item 
  •   DSpace Home
  • TIP FAKÜLTESİ
  • Makale
  • Web of Science İndeksli Yayınlar
  • View Item
  •   DSpace Home
  • TIP FAKÜLTESİ
  • Makale
  • Web of Science İndeksli Yayınlar
  • View Item
JavaScript is disabled for your browser. Some features of this site may not work without it.

Evaluation of blood-testis barrier integrity in terms of adhesion molecules in nonobstructive azoospermia

Thumbnail
Date
2020
Author
Aydin, Sevim
Billur, Deniz
Kizil, Sule
Ozkavukcu, Sinan
Celikkan, Ferda Topal
Aydos, Kaan
Erdemli, Esra
xmlui.mirage2.itemSummaryView.MetaData
Show full item record
Abstract
Blood-testis barrier (BTB) is critical for maintaining fertility. The integrity of tight junctions (TJs) provides restricted permeability of BTB. The aim of this study was to evaluate the relationship between BTB and Sertoli cells. Testicular sperm extraction (TESE) obtained from nonobstructive azoospermia (NOA) patients was examined: Group I (spermatozoa+) and Group II (spermatozoa-). The tissues were stained with haematoxylin eosin, periodic acid-Schiff and Masson's trichrome for Johnsen's score evaluation. Apoptosis and adhesion molecules such as claudin-11, occludin and ZO-1 were assessed. In Group I, the integrity of the seminiferous tubules was intact. In Group II, some seminiferous tubule walls were lined only with Sertoli cells, had a thickening of the basement membrane, and oedema in interstitial spaces. In Group I, the seminiferous tubule consisted of a stratified columnar epithelium, claudin-11 expressions were observed as linear staining in the basal zone of the tubule, while seminiferous tubules, with low epithelium, displayed a punctate type of staining. Immunohistochemical observations were consistent with the ultrastructural findings. In Group II, high apoptosis and unstained/irregular TJ formation in claudin-11, occludin and ZO-1 were observed. In conclusion, disruption of relation between BTB and TJs may reveal inadequate spermatogenesis, which is one of the mechanisms behind azoospermia.
URI
http://hdl.handle.net/20.500.12591/205
xmlui.mirage2.itemSummaryView.Collections
  • Web of Science İndeksli Yayınlar
  • WOS İndeksli Yayınlar Koleksiyonu





Creative Commons License
DSpace@LokmanHekim by Lokman Hekim University Institutional Repository is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 4.0 Unported License..

DSpace software copyright © 2002-2016  DuraSpace
Contact Us | Send Feedback
Theme by 
Atmire NV
 

 



About HUAES
Open Access PolicyOpen Access InstructionGuidesSubcriptionsContact
sherpa/romeo

Browse

All of DSpaceCommunities & CollectionsBy Issue DateAuthorsTitlesSubjectsThis CollectionBy Issue DateAuthorsTitlesSubjects

My Account

LoginRegister

DSpace software copyright © 2002-2016  DuraSpace
Contact Us | Send Feedback
Theme by 
Atmire NV