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dc.contributor.authorZeybek, Naciye Dilara
dc.contributor.authorSur, Ünzile
dc.contributor.authorOflaz, Ofcan
dc.contributor.authorErkekoğlu, Pınar
dc.contributor.authorBalcı, Aylin
dc.contributor.authorÖzkemahlı, Gizem
dc.contributor.authorAşçı, Ali
dc.contributor.authorKızılgün, Murat
dc.contributor.authorEdebal, Oğuz Han
dc.contributor.authorKoçer-Gümüşel, Belma
dc.description.abstractAroclor 1254 (A1254), a mixture of polychlorinated biphenyls, exerts hepatic, renal, and reproductive toxicity in rodents. This study aimed to determine a protective role of selenium on histopathological changes, oxidative stress, and apoptosis caused by A1254 in rat kidney. It included a control group, which received regular diet containing 0.15 mg/kg Se (C), a Se-supplemented group (SeS) receiving 1 mg/kg Se, a Se-deficient group (SeD) receiving Se-deficient diet of ≤0.05 mg/kg Se, an A1254-treated group (A) receiving 10 mg/kg of Aroclor 1254 and regular diet, an A1254-treated group receiving Se-supplementation (ASeS), and an A1254-treated group receiving Se-deficient diet (ASeD). Treatments lasted 15 days. After 24 h of the last dose of A1254, the animals were decapitated under anaesthesia and their renal antioxidant enzyme activities, lipid peroxidation (LP), glutathione, protein oxidation, and total antioxidant capacity levels measured. Histopathological changes were evaluated by light and electron microscopy. Apoptosis was detected with the TUNEL assay. Kidney weights, CAT activities, and GSH levels decreased significantly in all A1254-treated groups. Renal atrophic changes and higher apoptotic cell counts were observed in the A and ASeD groups. Both groups also showed a significant drop in GPx1 activities (A - 34.92 % and ASeD - 86.46 %) and rise in LP (A - 30.45 % and ASeD - 20.44 %) vs control. In contrast, LP levels and apoptotic cell counts were significantly lower in the ASeS group vs the A group. Histopathological changes and renal apoptosis were particularly visible in the ASeD group. Our findings suggest that selenium supplementation provides partial protection against renal toxicity of Aroclor 1254.en_US
dc.publisherEzcacılık Fakültesien_US
dc.rightsAttribution-NonCommercial-NoDerivs 3.0 United States*
dc.subjectSprague-Dawley ratsen_US
dc.subjectTUNEL assayen_US
dc.subjectantioxidant enzymesen_US
dc.subjectelectron microscopyen_US
dc.subjectoxidative stressen_US
dc.subjectpolychlorinated biphenylsen_US
dc.subjectultrastructural changesen_US
dc.titleRenal changes and apoptosis caused by subacute exposure to Aroclor 1254 in selenium-deficient and selenium-supplemented rats.en_US
dc.contributor.departmentFarmasötik Toksikolojien_US

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