In vitro analysis of the renin-angiotensin system and inflammatory gene transcripts in human bronchial epithelial cells after infection with severe acute respiratory syndrome coronavirus
Date
2020Author
Turk, Can and Turk, Seyhan and Temirci, Elif Sena and Malkan, Umit Yavuz
and Haznedaroglu, Ibrahim C.
xmlui.mirage2.itemSummaryView.MetaData
Show full item recordAbstract
Introduction:
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is a
recently identified coronavirus family member that triggers a
respiratory disease similar to severe acute respiratory syndrome
coronavirus (SARS-CoV). SARS-CoV and SARS-CoV-2 are very similar to each
other in many respects, such as structure, genetics, and pathobiology.
We hypothesized that coronaviruses could affect pulmonary tissues via
integration with the critical immune genes after their interaction with
renin-angiotensin system (RAS) elements. The aim of the present
bioinformatics study was to assess expression changes of the RAS and
non-RAS genes, particularly immune response genes, in the lung
epithelial cells after infection with SARS-CoV.
Methods:
Linear regression, hierarchical clustering, pathway analysis, and
network analysis were performed using the E-GEOD-17400 data set.
Results:
The whole-genome expression data of the lung epithelial cells infected
with SARS-CoV for 12, 24, and 48 hours were analyzed, and a total of 15
RAS family and 29 immune genes were found to be highly correlated with
the exposure time to the virus in the studied groups.
Conclusion:
RAS genes are important at the initiation of the infections caused by
coronavirus family members and may have a strong relationship with the
exchange of immune genes in due course following the infection.
xmlui.mirage2.itemSummaryView.Collections

DSpace@LokmanHekim by Lokman Hekim University Institutional Repository is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 4.0 Unported License..